Figure 2.

Topoisomerase II transports DNA with a reduced rate when it cannot hydrolyze ATP. Results from single turnover DNA decatenation time courses with either 50 nM E66Q enzyme and 1 mM ATP (○), or 50 nM wt enzyme and 1 mM AMPPNP (□) are shown. For these particular reactions, rate constants for decatenation are 0.39 ± 0.07 s⁻¹ for E66Q and ATP, and 0.32 ± 0.04 s⁻¹ for wt and AMPPNP.