Figure 3.

Hydrolysis of one ATP is sufficient to accelerate DNA transport. Single turnover DNA decatenation time course results are shown for reactions with 1 mM ATP and 25 nM DNA and either 50 nM wt/wtΔC-his (○), 50 nM wt/wtΔC-his plus 100 μM freshly prepared vanadate (□), or 50 nM E66Q/wtΔC-his (▵); rate constants, calculated by fitting data from three separate integrations of the gels, are 6.1 ± 0.5 s⁻¹, 5.9 ± 0.7 s⁻¹, and 4.7 ± 0.7 s⁻¹, respectively.