Figure 1.

The amino-terminal and carboxyl-terminal domains of NRSF are sufficient to mediate transcriptional repression. Various regions of NRSF, depicted schematically on the left with amino acid residue numbers, were fused in-frame to GAL4-DBD. The resultant series of GAL4 DBD-NRSF (G4-NR) constructs was cotransfected into Neuro2a cells with a luciferase reporter plasmid, pGL-S10PR5GB, containing the SCG10 promoter flanked by five copies of the GAL4-binding site linked to a luciferase gene, together with pRL-TK as an internal control.