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. 1999 Nov 23;96(24):13691–13696. doi: 10.1073/pnas.96.24.13691

Figure 4.

Figure 4

Inhibition of HDAC activity by TSA relieves transcriptional repression by RD-1 but not that by RD-2. (A) TSA relieves transcriptional repression mediated by the N-terminal (a) but not by the C-terminal (b) domain. Neuro2a cells were transfected with plasmids expressing either GAL4DBD-NRSF-N-terminal (G4-NR 1–156) or GAL4DBD-NRSF-C-terminal (G4-NR 989-1097) together with a luciferase reporter plasmid pGL-S10PR5GB. After 24 hr, the cells were treated with various concentrations of TSA (0–200 ng/ml) for an additional 24 hr. Relative luciferase activities are calculated relative to the activity obtained by using the GAL4DBD plasmid as an effector. (B) TSA relieves NRS-mediated transcriptional repression by NRSF. Neuro2a cells were transfected with the NRSF expression plasmid pCI-NRSF and the NRS-containing reporter plasmid pGL-S10PRS36+ and treated as described above.