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tPA activity in DHB4 (wild type), WP597 (trxB), FA112 (trxB gshA supp), and FA113 (trxB gor supp) monitored by the fibrinolysis assay. Cells were transformed with plasmids pTrcvtPA, and pFA5 (bottom row only). Soluble protein (10 μg) from induced cultures was spotted onto fibrin/agarose plates and was incubated for 24 hr at 37°C. Clearance zones qualitatively measure biological activity of bacterially produced vtPA.
