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Pulse–chase of alkaline phosphatase. Signal sequenceless alkaline phosphatase (AP) was immunoprecipitated and separated by native PAGE, such that the oxidized form (ox) was distinguished from the reduced form (red). Time is indicated in minutes postchase. Strains used were DHB4 (wild type), WP597 (trxB), and FA113 (trxB gor supp) transformed with plasmid pAID135. OmpA was used as an internal standard.
