Table 1.
Plasmids and strains employed in this study
| Plasmid name | Relevant features | Source |
|---|---|---|
| pAID135 | PhoA(Δ2-22) under control of tac promoter | Ref. 18 |
| pΔA-uPa | ΔA-uPA under control of tac promoter | Ref. 14 |
| pFA2 | GrxA in pBAD33 under its own RBS | This work |
| pFA3 | TrxA in pBAD33 under its own RBS | Ref. 19 |
| pFA4 | TrxA(P34S) in pBAD33 | This work |
| pFA5 | TrxA(G33P,P34Y) in pBAD33 | Ref. 19 |
| pfA6 | TrxA(G33P,P34H) in pBAD33 | Ref. 19 |
| pFA7 | TrxA(P34H) in pBAD33 | Ref. 19 |
| pFA8 | TrxA(C35A) in pBAD33 | Ref. 19 |
| pFA21 | TrxB in pBAD33 under its own RBS | This work |
| pFA22 | trxB upstream region in pFA21 at NsiI site | This work |
| pFA28 | trxB upstream region, araC, ParaB-trxB in pKOV | This work |
| pBADdsbA | DsbA in pBAD33 with optimized RBS | This work |
| pBADdsbC | DsbC in pBAD33 with optimized RBS | This work |
| pBADΔSSdsbA | DsbA(Δ2-19) in pBAD33 with optimized RBS | This work |
| pBADΔSSdsbC | DsbC(Δ2-20) in pBAD33 with optimized RBS | This work |
| pBADrPDI | mature rat PDI in pBAD33 with optimized RBS | This work |
| pTrcStIItPA | human tPA with StII leader in pTrc99A | Ji Qiu, University of Texas |
| pTrcStIIvtPA | tPA(Δ6-175) with StII leader in pTrc99A | This work |
| pTrctPA | tPA in pTrc99A | This work |
| pTrcvtPA | tPA(Δ6-175) in pTrc99A | This work |
| pBAD33 | araBAD promoter, CmR, pACYC ori | Ref. 16 |
| pTrc99A | trc promoter, AmpR, ColE1 ori | Amersham Pharmacia |
| pKOV | counter-selectable allele exchange vector | G. Church laboratory, Harvard University |
| Strain | Genotype | Source |
| DHB4 | MC1000 ΔphoA(PvuII) phoR ΔmalF3 F′[lac+(lacIQ) pro] | Ref. 15 |
| WP597 | DHB4 trxB::Km | W. Prinz, Harvard University |
| FA112 | DHB4 gshA20::Tn10Km trxB::Km… Tn10 supp | This work |
| FA113 | DHB4 gor522…mini-Tn10Tc trxB::Km supp | This work |
| FA196 | DHB4 ΔtrxB::ParaB-trxB | This work |
| FA222 | FA196 gor522…mini-Tn10Tc | This work |