Table 1.
Cell Type | Treatment | Transmonolayer resistance* (Ohms × cm2) |
31E-pBabeVector | w/o Wnt-3a | 290 +/- 22 |
+ Wnt-3a | 620 +/- 36 | |
31E-pBabeSFRP-4 | w/o Wnt-3a | 250 +/- 18 |
+ Wnt-3a | 310 +/- 27 |
* Cultures previously selected with puromycin were grown on nitrocellulose filter inserts (2.2 cm diameter) for 48 hrs prior to adding treatment with or without Wnt-3a at 5 μl/ml for a further 28 hrs. Values shown are the mean +/- S.E.M. of 5 measurements made on parallel inserts taken across the membrane from apical to basal sides with the Millicell ERS instrument.