Table 3.
McDonald-Kreitman test comparing the ratio of nonsynonymous to synonymous differences within Chlamydomonas reinhardtii to that found between C. reinhardtii and Chlamydomonas incerta.
| Polymorphisms within C. reinhardtii | Substitutions between C. reinhardtii and C. incerta | NI | G | P | |||
|---|---|---|---|---|---|---|---|
| mtDNA | Protein-codinga | Nonsynonymous | 3 | 61 | 0.653 | 0.533 | 0.465 |
| Synonymous | 36 | 478 | |||||
| rtl | Nonsynonymous | 5 | 111 | 0.746 | 0.185 | 0.667 | |
| Synonymous | 4 | 119 | |||||
| Nuclear DNA | Exonic (overall)b | Nonsynonymous | 1 | 18 | 0.159 | 4.837 | 0.027 |
| Synonymous | 21 | 60 | |||||
| CBLP | Nonsynonymous | 0 | 0 | undef | undef | undef | |
| Synonymous | 1 | 1 | |||||
| PETC | Nonsynonymous | 0 | 3 | 0.000 | undef | undef | |
| Synonymous | 2 | 4 | |||||
| PDK | Nonsynonymous | 0 | 2 | 0.000 | undef | undef | |
| Synonymous | 3 | 18 | |||||
| MAT3 | Nonsynonymous | 1 | 9 | 0.677 | 1.081 | 0.298 | |
| Synonymous | 6 | 18 | |||||
| SFA | Nonsynonymous | 0 | 4 | 0.000 | undef | undef | |
| Synonymous | 9 | 19 |
Note--NI, neutrality index (ratio of nonsynonymous to synonymous polymorphisms within C. reinhardtii compared to the ratio of nonsynonymous to synonymous fixed differences between C. reinhardtii and C. incerta); G, G-test of independence (determines if the proportion of nonsynonymous substitutions is independent of whether the substitutions are fixed or polymorphic); P, probability of G-test; undef, undefined. C. incerta data came from [6] and [7]. Note: in no case was the McDonald-Kreitman test statistically significant.
a Includes all protein coding genes excluding rtl.
b Concatenated exons from all 5 nuclear genes.