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Parameter settings for Sanger and 454 tests
| Minimum Hit Length | Minimum Hit Identity | Maximum e-value | Required Length of MCS | Required MCS Coverage Depth | Minimum Number of Hits for Extension | |
| Sanger | 150 | 89 | 1.00E-25 | 150 | 3 | 2 |
| 454 | 30 | 88 | 1.00E-10 | 30 | 3 | 2 |
| Required Coverage Length | Maximum Extend Length | Minimum BL2SEQ Hit Size | Maximum BL2SEQ e-value | Maximum Number of Times a Sequence Can Be Used in One Direction | Other | |
| Sanger | 150 | 50 | 90 | 1.00E-10 | 13 | |
| 454 | 30 | 40 | 15 | 10 | 4 | BL2SEQ Word Size: 7 |
