Table 2.
Assay conditions for each of the nine genes evaluated.
| Gene symbol | Primers 5'-3' (forward, reverse) | Amplicon length, bp | PCR efficiency, % | Correlation with dilution series (R2) |
| ACTB | GGACCTGACGGACTACCTC CACGCACGATTTCCCTCTC |
83 | 95.2 | 0.999 |
| B2M | CCTGCTCGGGCTACTCTC CATTCTCTGCTGGGTGACG |
89 | 100.2 | 1.000 |
| GAPDH | ATCTGACCTGCCGCCTGGAG CGATGCCTGCTTCACCACCTTC |
68 | 102.6 | 1.000 |
| HPRT | AATTATGGACAGGACTGAACGG ATAATCCAGCAGGTCAGCAAAG |
121 | 93.9 | 1.000 |
| R18S | GTCTGCCCTATCAACTTTCG TTCCTTGGATGTGGTAGCC |
119 | 94.1 | 0.998 |
| RPL32 | GGGAGCAATAAGAAAACGAAGC CTTGGAGGAGACATTGTGAGC |
138 | 97.0 | 1.000 |
| SDHA | GAGGAATGGTCTGGAATACTG GCCTCTGCTCCATAAATCG |
91 | 96.0 | 0.999 |
| TFRC | TGGCTACTTGGGCTATTGTAAACG GGTGGTTCTGTTCCCTCTATCTCC |
90 | 97.6 | 0.998 |
| UBB | TTCGTGAAGACCCTGACC CCTTATCCTGGATCTTGGC |
91 | 99.4 | 0.999 |