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. 1999 Nov 23;96(24):13714–13719. doi: 10.1073/pnas.96.24.13714

Figure 2.

Figure 2

Expression analysis of recombinant peppermint and E. coli IPK, and partial purification of the E. coli IPK. SDS/PAGE lanes are: 1, molecular mass markers; 2, peppermint IPK expressed from pBAD-MPK in E. coli BL21-CodonPlus-RIL cells (induction with 0.02% arabinose); 3, E. coli background control (pBAD-MPK in E. coli BL21-CodonPlus-RIL cells (repression with 0.02% glucose); 4, E. coli IPK expressed from pBAD-ECK in E. coli Top 10 One Shot cells (induction with 0.02% arabinose); and 5, partially purified E. coli IPK (from 4 above) after affinity chromatography.