Table 1.

Substrate specificity of recombinant IPKs

Substrate	Rate,* pmol⋅(s⋅g protein)−1
	Peppermint†	E. coli† (repressed)	E. coli† (induced)	E. coli (partially purified)
IP	1.43	<1.0‡	2.41	178  ±  81
ISO	0.10	0.08	0.10	79  ±  24
DMAP	ND	ND	ND	ND
DMA	<0.01	<0.01	<0.01	11  ±  5
DXP	<0.1§	<0.1§	<0.1§	ND
Deoxyxylulose	<0.1§	<0.1§	<0.1§	ND
MEP	<0.2§	<0.2§	<0.2§	ND
MVA	ND	ND	ND	ND

ND, not detectable. 

^*Rates are given as averages of two to five separate experiments, with SD where appropriate. 

^† These crude extracts contained high phosphatase activity; the rates given are thus minimums. 

^‡ The kinase activity in this assay varied but was always below the listed rate. 

^§ In most of these assays, no kinase activity was observed; in some cases, minor levels of the apparent product were detected.