Table 1. IFN-γ secretion by peptide-specific T cell lines in response to stimulation by antigen presenting cells loaded with MEP-mel protein.

DC or antigen presenting LCL721.221cells were loaded with MEP-mel protein or with peptides, and coincubated overnight with peptide-specific T cells. MUC1 and MUC1-specific T cells served as negative control. IFN-γ levels secreted into the supernatant were determined.

APC	Loading	T-cell specificity (IFN-γ secretion, pg/ml)
DC		Gp209	Mart1	MUC1
	None	66	68	49
	MEP-mel	235	135	59
	Specific peptide	5840	7220	16460
	Control peptide	140	53	43
LCL	None	41	46	297
	MEP-mel	83	125	69
	Specific peptide	5793	5253	14360
	Control peptide	81	64	406