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. 2008 Jun 9;205(6):1277–1283. doi: 10.1084/jem.20080162

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© 2008 Plitas et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — TLR9^−/− mice have increased lymphocyte infiltration and decreased local inflammatory cytokine production after CLP. 12 h after CLP or sham laparotomy, the numbers of viable splenocytes (A) and infiltrating peritoneal lymphocytes (B) were counted on a hemocytometer (BrightLine; Hausser Scientific) after Trypan blue staining (three to five mice per group). Peritoneal cells are expressed per milliliter of peritoneal fluid. (C) Peritoneal cells pooled from three to five mice 12 h after CLP were cultured without restimulation for 24 h, and the supernatant cytokine levels were determined with a cytometric bead array. Peritoneal cells from mice that underwent sham laparotomy secreted a minimal amount of cytokines (not depicted). Data shown are means of values ± SEM obtained from individual mice (except for cells pooled in C) and are representative of at least two independent experiments. *, P < 0.05.