Figure 2.

H-CDR3 spectratypes of the V_H3.15 and V_H6 transcripts expressed by the splenic B cell subsets of an 8-mo-old child. (A) Two different marker combinations were used to isolate splenic GC (CD19⁺CD24⁻CD38⁺) and transitional B cells (CD19⁺CD38⁺⁺CD24⁺⁺) or naive IgD⁺CD27⁺, IgD⁺CD27⁺ and switched IgD⁻CD27⁺ B cells. (B) Spectratyping was performed on V_H3.15 and V_H6 μ and/or γ transcripts from each cell fraction, as described in the legend of Fig. 1. Peaks identified by an asterisk were further sequenced to evaluate intrapeak clonal diversity.