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. 2008 Jun 9;205(6):1331–1342. doi: 10.1084/jem.20071555

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© 2008 Weller et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Detectable AID expression in splenic IgD⁺CD27⁺ cells from very young children. Relative AID and Bcl-6 expression levels were determined for various splenic B cell subsets from different donors. AID and Bcl-6 sequences were amplified by real-time quantitative PCR from cDNA from IgD⁺CD27⁺, IgD⁺CD27⁻ (naive), and IgD⁻CD27⁺ cells purified by two consecutive cell sortings. The results shown were obtained from two independent PCRs (except for D6), each performed in triplicate. The relative expression of AID or Bcl-6 in each subset was calculated by the comparative method, normalizing to 1 the expression of AID in the IgD⁺CD27⁺ fraction and of Bcl-6 in the naive subset of the 8-mo-old donor (D3). PCRs with a threshold cycle (Ct) >35 were considered NS.