Skip to main content
. 2008 Jun 9;205(6):1343–1355. doi: 10.1084/jem.20071572

Figure 2.

Figure 2.

Direct maturation of AA4.1+ immature B cells to AA4.1 intestinal B cells. (A) Intestinal inflammation was induced by administration of 4% DSS in drinking water and the treatment was terminated by changing the DSS-containing water to normal water at day 4. Expression of AA4.1 versus B220 (top) in total large intestinal cells (day 8) and CD23 versus IgM expression on the gated AA4.1+ B220+ cells (bottom; n = 12) is shown. (B) 1 mg BrdU was administered (intraperitoneally) into intact WT mice twice with a 24-h interval between injections, and the mice were killed at 2 and 6 d after initial BrdU injection. The B220 versus BrdU expressions on the gated AA4.1+ (top) or AA4.1 (bottom) IgM+ cells from large intestine are shown. The data are representative of three individual experiments. (C and D) Purified AA4.1+ B220+ B cells from the spleen of GFP transgenic mice (left) were intravenously transferred into WT mice with or without prior DSS exposure. 6 d after cell transfer, the recipient mice were killed. GFP- and IgM-expressing B cells in the recipient colon and spleen were identified (C). The expression of CD21 and CD23 versus IgM on the gated GFP+ cells in the spleen and colon of recipient mice without DSS treatment is shown in D. The data are representative of three to six individual recipients/group. (E and F) Purified IgDhigh B cells from the spleen of GFP transgenic mice were intravenously transferred into WT mice that had being treated without (top) or with DSS (bottom). 4 d after cell transfer, the recipient mice were killed. The expressions of GFP versus IgM in recipient spleen (left) and colon (right) are shown in (E). The expressions of CD21 or CD23 on the gated GFP+ cells from the spleen (left) and large intestine (right) of DSS treated mice are shown in (F). The data are representative of three individual recipients.