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. 2008 Jun 9;205(6):1381–1393. doi: 10.1084/jem.20080034

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© 2008 Lochner et al.

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Figure 1. — Diversity and distribution of RORγt⁺ T cells in vivo. (A and B) Flow cytometry analysis of cells isolated from the organs of 8–12-wk-old Rorc(γt)-Gfp^TG mice. Plots are gated on CD3⁺ cells (A) or GFP⁺ CD3⁺ cells (B). Numbers indicate mean percent cells in quadrants ± SD obtained with at least three Rorc(γt)-Gfp^TG mice. LPLs, lamina propria lymphocytes isolated from small intestine; mLN, mesenteric LNs; BM, bone marrow. (C) Immunofluorescence histology of RORγt⁺ cells in the small intestine of Rorc(γt)-Gfp^TG mice. Most RORγt⁺ cells in villi are T cells, whereas RORγt⁺ cells in cryptopatches located between crypts are CD3⁻ LTi cells. Bar, 50 μm. (D) Expression of CD4 and CD8α by spleen GFP⁺TCR-β⁺ and lung or GFP⁺TCR-δ⁺ cells.