Figure 3.
RORγt+ T cells express genes involved in the Th17 or T reg cell pathway. (A) Cells isolated from the spleen and mesenteric LNs of Rorc(γt)-GfpTG mice were sorted into eight distinct populations based on their expression of GFP, CD3, TCR-β, TCR-δ, CD4, and CD25 (Fig. S1), and gene expression was assessed using real-time PCR. Ct values were normalized to the mean Ct of five housekeeping genes. Data are the mean of two or three independent experiments. (B) Foxp3+ RORγt+ T cells express IL-10. Cells isolated from LNs of Rorc(γt)-GfpTG mice were restimulated in vitro with PMA/ionomycin for 5 h and subjected to intracellular staining for GFP, IL-17, Foxp3, and IL-10 or an isotype control. Numbers indicate percent cells in quadrants. Results are representative of at least three individual experiments.