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. 2008 Jun 9;205(6):1381–1393. doi: 10.1084/jem.20080034

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© 2008 Lochner et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Generation and differentiation of RORγt⁺ Tαβ cells in vitro. MACS-sorted naive (CD62L⁺) CD4⁺ T cells from the spleens of Rorc(γt)-Gfp^TG mice were stimulated in duplicates with anti-CD3ε and anti-CD28 in the presence of blocking anti–IFN-γ and anti–IL-4 antibodies and the indicated cytokines or RA. After different periods of time, cells were restimulated with PMA/ionomycin for 5 h and analyzed by flow cytometry for the expression of GFP, Foxp3, IL-17, and IL-10. All plots are gated on TCR-β⁺ cells, except plots for IL-10 that are gated on GFP⁺TCR-β⁺ cells. Numbers indicate percent cells in quadrants. Data are representative of three independent experiments.