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. 2008 Jun 9;205(6):1447–1461. doi: 10.1084/jem.20071450

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© 2008 Gerosa et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — A combination of NOD2 and TLR2 ligands mimics IL-23 and IL-12 induction by M. tuberculosis. mono-DCs treated with IFN-γ were labeled with [³⁵S]methionine (A and B) or unlabeled (C and D) and stimulated with different combinations of MDP, Pam2C, and Pam3C, with or without R848. After 18 h, supernatants were immunoprecipitated with anti–IL-12 p35, anti–IL-23 p19, or anti–IL-12/23 p40 mAbs and resolved in nonreducing SDS-PAGE (A and B), or were evaluated for IL-12 p75, IL-23, and IL-10 by ELISA (C). In D, cells were lysed at 3, 6, and 12 h, and mRNA accumulation was determined using the QuantiGene multiplex assay. Results in A and B are representative of those obtained in three independent experiments with cells derived from different donors. Data in C are mean ± SE values from mono-DCs derived from three different donors. Results in D were obtained with cells from two different donors and are expressed as the mean ± SD of triplicate cultures. Inverted triangles indicate not done.