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. Author manuscript; available in PMC: 2008 Jun 5.
Published in final edited form as: Cancer Cell. 2008 May;13(5):385–393. doi: 10.1016/j.ccr.2008.03.015

Figure 4. Knocking Down EGFR Leads to Downregulation of SGLT1, and Knocking Down SGLT1 Results in a Similar Cell Death Phenotype as Knocking Down EGFR.

Figure 4

(A) No changes on GLUT1 expression were detected by knocking down EGFR, but SGLT1 was downregulated to an undetectable level. β-actin served as a loading control.

(B) Cells treated with SGLT siRNA led to downregulation of SGLT1 after 4 days of culture in MEM.

(C) This treatment also led to a significant proportion of cell deaths (p < 0.01) as evidenced by the presence of sub-G1 cells (arrow), and hgMEM was able to prevent cells from death. (The flow cytometry data of control vector-transfected cells were similar to those of the control cells used in the EGFR siRNA experiment in Figure 2.)

(D) As with the EGFR siRNA-treated cells, cells that underwent SGLT1 siRNA treatment also showed a large amount of autophagosomes (arrows) on transmission electron microscopy (bar, 2 μm).