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. Author manuscript; available in PMC: 2008 Jun 5.
Published in final edited form as: Cancer Cell. 2008 May;13(5):385–393. doi: 10.1016/j.ccr.2008.03.015

Figure 6. Rescue Cells Treated with EGFR siRNA from Death by Re-Expression of WT-EGFR or kmtEGFR.

Figure 6

(A) PC-3MM2 cells were treated with siRNA targeting the 5′UTR region of EGFR mRNA, which downregulated the EGFR level after a 4-day siRNA treatment. Both WT-EGFR and kmtEGFR were re-expressed in the siRNA-treated cells and were able to upregulate SGLT1 expression. The absence of kinase activity of kmtEGFR was shown by western blotting with an anti-pEGFR antibody. β-actin served as a loading control.

(B) The re-expression of WT-EGFR and kmtEGFR also rescued the EGFR siRNA-treated cells from death. The sub-G1 proportion is indicated by the arrows. (Duplicate samples were used in each group of samples.)