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Primary Jα usage in TEA and Jα49 promoter-deleted mice. Vα8 to Cα RT-PCR products obtained from thymocytes of 4-5 week old Rorc-/-, ΔJ49Rorc-/- and ΔTEAΔJ49Rorc-/- mice were cloned and sequenced to evaluate Jα usage. The fraction of clones using particular Jα segments is plotted. Numbers of clones analyzed were 72, 45 and 66 for Rorc-/-, ΔJ49Rorc-/- and ΔTEAΔJ49Rorc-/-, respectively.
