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. Author manuscript; available in PMC: 2008 Jun 5.
Published in final edited form as: Nat Immunol. 2005 Apr 3;6(5):481–489. doi: 10.1038/ni1189

Figure 5.

Figure 5

Quantitative Southern blot analysis of Jα rearrangement on a ΔTEAΔJ49 allele. (a) Thymocyte (T) and kidney (K) genomic DNA was isolated from B6:ΔTEAΔJ49 mice and MscI (M), SpeI (Sp), or SacI (Sa) plus SpeI digests were analyzed by Southern blot using Jα61-60, Jα48-47 and Jα40-39 probes (filled rectangles) and a Cα probe. (b) Retention of germline fragment was calculated by first normalizing hybridization signals for Jα to Cα in thymocytes and in kidney. Normalized thymus signals were then expressed as a percentage of the normalized kidney signals. The results represent the mean ± SEM of triplicate determinations.