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. Author manuscript; available in PMC: 2008 Jun 5.
Published in final edited form as: Nat Immunol. 2005 Apr 3;6(5):481–489. doi: 10.1038/ni1189

Figure 7.

Figure 7

Germline JαCα transcription in TEA and Jα49 promoter-deleted mice. (a) RNA isolated from ΔTEAΔJ49Rxβ thymocytes was subjected to 5′ RACE using a Cα primer. The relative frequencies of RACE clones reflecting initiation upstream of or within individual Jα segments is plotted (22 total). Three of five clones identified as Jα37 initiated 66-105 bp downstream of Jα37 and spliced to Cα using a 5′ splice site 129 bp downstream of Jα37. (b,c) Serial three-fold dilutions of cDNA (wedges) prepared from thymocytes of the indicated genotypes were analyzed by PCR to detect JαCα transcripts, total Cα-containing transcripts, or Actb transcripts. (-) indicates a control PCR lacking cDNA. PCR reactions were analyzed by Southern blot using Cα or Actb probes. The results are representative of two experiments.