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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 Jun 29;293(3):L790–L799. doi: 10.1152/ajplung.00099.2007

Fig. 1.

Fig. 1

Transforming growth factor-β1 (TGF-β1) stimulation upregulates human angiotensin II type 1 receptor (hAT1R) steady-state mRNA levels in human fetal pulmonary fibroblasts (hPFBs) in a time-dependent manner. hPFBs were grown to 70–80% confluence, washed twice, and serum-starved for 24 h. A: total RNA was isolated from TGF-β1-treated (4 ng/ml) fibroblasts at the times indicated. RNA (20 μg) was fractionated, blotted, and probed with a radiolabeled cDNA specific for hAT1R mRNA. The Northern blot was stripped and reprobed with a GAPDH control cDNA to ensure that the relative quantity of the total RNA present in each lane was approximately equal. Data are representative of 3 separate experiments. B: TGF-β1 time course of hAT1R and GAPDH Northern hybridization signal intensity was quantitated by densitometric analysis. Each point represents the relative hybridization signal (±SE) normalized to 0-h treatment with vehicle (100%) from 3 separate experiments. *P < 0.01, TGF-β1-treated vs. nontreated hPFBs.