Fig. 2.

RNA gel blot analysis of expression of CaPMEI1, CaSAR82A and CaBPR1 in pepper plants. Membranes were hybridized with probes from the 3′ UTR region of pepper CaPMEI1 cDNA or full-length CaBPR1 cDNA. Equal loading (20 μg) was verified by visualizing RNA on a gel stained with ethidium bromide. H healthy, M mock-inoculated or mock-treated. aCaPMEI1 expression in various organs of pepper plants. b Expression of CaPMEI1 and CaBPR1 in pepper leaves at various time intervals after inoculation with C. coccodes or virulent strain Ds1 (compatible interactions with pepper: susceptible response) and avirulent strain Bv5-4a (incompatible interactions with pepper: resistant response) of Xcv.cCaPMEI1 expression in lower (local) infected and upper (systemic) uninfected leaves at various time intervals after inoculation with the virulent and avirulent strains Ds1 and Bv5-4a of Xcv, P.fluorescence ATCC13525 and E.coli JM109. The lower leaves of pepper plants were inoculated at the 6-leaf stage. For the mock-inoculation, the lower leaves were infiltrated with 10 mM MgSO₄. d Expression of CaPMEI1 and CABPR1 in pepper leaves at various time intervals after treatment with salicylic acid (SA, 5 mM), methyl jasmonate (MeJA, 100 μM), ethylene (5 μl L⁻¹) and abscisic acid (100 μM). e Expression of CaPMEI1 and CaSAR82A in pepper leaves at various time intervals after treatment with drought, wounding, cold and H₂O₂