In situ localization of CaPMEI1 transcripts in pepper leaf and stem tissues. Cross sections of leaf tissues were hybridized with CaPMEI1 antisense (a, c, e, g) and sense (b, d, f, h) DIG-labeled RNA probes, and then photographed under bright-field conditions. The transcript signal is purple. a, b Untreated leaf tissues. c, d Untreated stems. e, f Leaf tissues at 24 h after inoculation with C. coccodes. g, h Leaf tissues treated with 5 μl L−1 ethylene. P phloem, X xylem, UE upper epidermis, LE lower epidermis, Vs vascular bundle, C cortical cell