Fig. 5.

Assay of thioredoxin–CaPMEI1 fusion protein antimicrobial activity. a Inhibitory effects of the CaPMEI1–thioredoxin fusion protein on mycelial growth of the plant pathogenic fungi F. oxysporum f.sp. matthiolae, A. brassicicola and B. cinerea. In each plate, the upper wells (a) were treated with purified thioredoxin and the lower wells (b) were treated with the purified thioredoxin-CaPMEI1 fusion protein. b Inhibition of germination and hyphal growth of F.oxysporum f.sp. matthiolae. Fungal spores were allowed to germinate and grow in 100 μL potato dextrose broth medium alone (top), or with 500 μg mL⁻¹ thioredoxin (middle) or 500 μg mL⁻¹ thioredoxin–CaPMEI1 fusion protein (bottom). Photographs were taken after incubation for 10 h at 28°C. Bars 20 μm. c Inhibition of spore germination and hyphal growth of F.oxysporum f.sp. matthiolae by CaPMEI1. The percentage of germinated spores and the length of fungal hyphae were determined by light microscopy. Data represent means ± SD from three independent experiments