Fig. 8.

Responses of wild-type (Col-0) Arabidopsis and CaPMEI1-OX transgenic plants to infection with P. syringae pv. tomato DC3000. a RNA gel blot analysis confirming CaPMEI1 overexpression (OX) in the transgenic Arabidopsis lines. Total RNA (10 μg) was loaded into each lane. The 3′ UTR region of pepper CaPMEI1 cDNA was used as a probe. b Growth of Pst DC3000 in the leaves of wild-type and transgenic plants. The mature leaves of the 6-week-old plants were infiltrated with a Pst Dc3000 suspension (10⁵ cfu mL⁻¹), and the degree of bacterial growth was rated at 0, 2 and 4 days after inoculation. c Disease symptoms on leaves of 6-week-old plants infiltrated with virulent Pst DC3000 (10⁵ cfu mL⁻¹). d Expression of pathogen-related (PR) genes in transgenic plants. Northern blot analyses were performed with 10 μg total RNA prepared from 5-week-old leaves of the wild-type (WT), vector control (smGFP) and transgenic (CaPMEI1::smGFP) plants. The samples were collected at 5, 15 and 25 h following pathogen infiltration with a suspension of the virulent strain Pst DC3000 (10⁵ cfu mL⁻¹)