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Efficiency of SSR marker development in C. moschata
| SSR development steps | Attrition | Total | % |
|---|---|---|---|
| Sequenced clones | 0 | 1,344 | 100 |
| SSR containing clones | 1 | 610 | 45.4 |
| Clones containing SSRs too close to one end | 2 | 35 | 2.6 (5.7 of 1) |
| Primer pairs designed and tested | 3 | 342 | 25.4 (56.0 of 1) |
| Working SSRs | 4 | 307 | 22.8 (89.8 of 3) |
| Polymorphic SSRs | 5 | 250 | 18.6 (81.4 of 4) |
