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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Horm Behav. 2008 Jan 10;53(5):673–692. doi: 10.1016/j.yhbeh.2007.12.013

Figure 12. Prenatal androgen exposure suppresses EB-induced PR mRNA expression in the POA of adult female rats.

Figure 12

Pregnant female Sprague-Dawley rats were subcutaneously injected with oil vehicle (V), 5mg of T, or 5mg of DHT daily on embryonic days 16–19.Female offspring were ovariectomized (OVX) in adulthood and treated with estradiol benzoate (EB; 30 μg sc) or sesame oil vehicle (oil). The POA was dissected from the brain of each animal, and RNA was isolated, reverse transcribed, and processed for PRA+B mRNA expression via semi-quantitative PCR. PRA+B mRNA expression is significantly increased in the POA of EB-treated, ovariectomized control rats (pV, n=6 for both groups) compared to their oil-treated counterparts (*p<0.05). PRA+B mRNA expression is not induced by EB in pT- (n=7 for both groups) or pDHT-treated (OVX/oil, n=7; OVX/EB, n=9) females. The semi-quantitative RT-PCR results for PRA+B are normalized to the housekeeping gene RPL19 and are represented as mean ± SEM. (From Foecking et.al., 2005)