Representative GnRH and LH release profiles of OVX, E2-primed rats (A) and OVX, E2-primed rats treated with ZK98299 (B). A, E2-priming stimulated significant increases in GnRH pulse amplitude commencing at approximately 1600 h, accompanied by a concomitant 3- to 5-fold increase in plasma LH. B, Treatment of E2-primed, OVX rats with ZK98299 prevented this E2-stimulated rise in both GnRH and LH release without affecting pulse frequency. Asterisks represent significant pulses as determined by the ULTRA pulse analysis program. (From Chappell et.al., 2000).