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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Horm Behav. 2008 Jan 10;53(5):673–692. doi: 10.1016/j.yhbeh.2007.12.013

Figure 8. Androgen treatment in adulthood suppresses progesterone receptor mRNA expression in the pituitary.

Figure 8

Cycling female rats were implanted with either empty Silastic capsules or capsules filled with either crystalline T, or DHT on proestrus. After four consecutive days of treatment, animals were sacrificed on presumptive proestrus and the pituitary of each animal was dissected. RNA was isolated from the pituitary, reverse transcribed, and processed for PRA+B mRNA expression via semi-quantitative PCR. PRA+B mRNA expression is significantly decreased in the pituitary of both the T- and DHT-treated rats compared to controls (n=5 for all groups). The semi-quantitative RT-PCR results for PRA+B are normalized to the housekeeping gene RPL19 and are represented as mean ± SEM.