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. 2008 Jun 5;118(7):2592–2599. doi: 10.1172/JCI33493

Figure 1. HemSCs express VEGF-Rs and mesenchymal cell marker CD90.

Figure 1

(A) Flow cytometric analysis of HemSCs, BM-MSCs, NHDFs, and HDMECs. Each cell type was grown in the EBM-2/20% FBS and assayed at passage 6. Gray histograms show cells labeled with FITC- or PE-conjugated antibodies. Black lines show isotype-matched control FITC- or PE-conjugated antibodies. Incubation with anti-CD31 and anti–VEGF-R2 was carried out following saponin permeabilization of HemSCs, BM-MSCs, and NHDFs. (B) RT-PCR analysis of Oct-4 and AML1 in 2 different HemSCs, Hem 106 and 109, with BM-MSCs and NHDFs shown for comparison. Ribosomal S9 (rS9) served as a control.