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The increased checkpoint activation by DSBs during DNA replication is dependent on Rad9 but not on Mrc1. Cultures of strains YCZ158 (ARS607::HOcs matHOcsΔ), YCZ200 (ARS607::HOcs matHOcsΔ mrc1Δ) and YCZ201 (ARS607::HOcs matHOcsΔ mrc1Δ) were grown in YPRaff and arrested in G1. HO was induced by shifting the culture to YPGal for 1 h, and cells were subsequently released from the G1 arrest. (A) Immunoblot and Rad53 autokinase analysis. (B) Flow cytometry analysis of DNA content.
