Figure 5.

Kinetics of mantADP binding to myosin V-1IQ and acto-myosin V-1IQ. Dependence of mantADP binding to myosin V-1IQ (○) or acto-myosin V-1IQ (●). The k_obs were determined from the fits of the time courses to single exponentials. (Inset) Time course of mantADP dissociation from acto-myosin V-1IQ (curve AM) and myosin V-1IQ (curve M). The rate constants for mantADP dissociation (k₋) determined from fits to single exponentials are 1.24 (± 0.01) s⁻¹ for myosin V-1IQ and 11.7 (± 0.2) s⁻¹ for acto-myosin V-1IQ. Fluorescence is normalized for clarity. Points are the average of 2–4 transients.