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. 2008 May 14;9:48. doi: 10.1186/1471-2199-9-48

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Copyright © 2008 Neely and Roberts; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Determination of Target for Methylation by M. BsaHI. Agarose gel showing conferred protection against cutting by M. BsaHI. Restriction digests are with R. BsaHI (Lanes 2–4), R. HhaI (Lanes 5–7) and R. HpaII (Lanes 8–10). For each restriction enzyme the three lanes show (from right to left) digest of unprotected DNA, digest of DNA incubated with M. BsaHI but without SAM and the digest of M. BsaHI protected DNA. Note the band that shifts from ~130 bases to ~170 bases in the R. HhaI digests (Lanes 6 and 7), indicating protection against cutting is conferred by M. BsaHI. Figures on the left show the size in bases of the bands in the DNA ladder (NEB 2-log ladder).