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. 2008 May 8;59(8):2125–2132. doi: 10.1093/jxb/ern075

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — Salicylic acid (SA) treatment followed by a heat shock (HS) leads to potentiation of Hsp/Hsc70 accumulation in seedlings. (A) The table represents relative Hsp/Hsc70 accumulation. Values represent the average of four biological repeats, SEM (P <0.001). (B) Representative western blot analysis of tomato seedlings, left untreated at room temperature (Control); or treated with SA (0.1 mM) for 17 h (SA); heat shocked at 40 °C for 30 min followed by a 2.5 h recovery (HS); or treated with both SA and HS (0.1 mM SA for 17 h followed by HS at 40 °C for 30 min) (HS+SA), using a mouse anti-Hsp/Hsc70 monoclonal antibody. SA alone did not induce Hsp/Hsc70 accumulation. SA in conjunction with HS was able to potentiate Hsp/Hsc70 accumulation significantly when compared with the HS treatment alone.