Table 1.

Prunus persica primers used for the real-time PCR LightCycler™ system

Target	Amplicon (bp)	Sequence (5′→3′)	Tm (°C)
Cobra (Cob)	314	Forward: ACT CAT CCA GGA AGC TGT GTA G	66
		Reverse: ATG GCT GTA TCA TTT ATT GTC GCA
Glucan synthase	337	Forward: TGG GAA ACA TGG TGG TAT GAG GA	66
		Reverse: CGA GAC ATT TGA AGT GAG TGA AC
Galactosyltransferase family protein	317	Forward: ATG TGA AAA GTG GAT GCG GAA TG	66
		Reverse: TTG GAT GAG AAG CGG GAA GAG A
Endopolygalacturonase (EndoPG)	544	Forward: GTC ATC TGG TGT CAC AAT C	54
		Reverse: ACC CTC AGT TGT TCC ATC
Cinnamoyl-CoA reductase (CCR)	312	Forward: ATC AAG TCC AAG ACC CCG AGA A	66
		Reverse: CGC CCA ACA CGG TGC CAG GA
Pectate lyase (PL)	294	Forward: GCC TTG CCG TAC GCT CAT GTC	65
		Reverse: CTT CAG CCT CAA CCC CTT CCC T
ER lumen protein retaining receptor 2 (ERD2)	317	Forward: GCC AGT ATT TTG GTC CTC CTT C	65
		Reverse: TCT TGA AAT GTG AAT TCC TCG TG
Coatomer protein gamma 2-subunit (gamma 2-COP)	350	Forward: AAT GGG CGA CCG TCC TTT TTA C	66
		Reverse: CAT CGA GCG ATC CAC ACT AGA T
Golgi transport protein SFT2-like	268	Forward: CAC TCA AAG GCC CGA AGA ATC A	66
		Reverse: CAA CGT CAC CTC CCA AAA CAT C
SNARE-like protein Vap 27-2	255	Forward: CCA AAG AAA TAC TGC GTG CGG C	65
		Reverse: GGA AGA GGG TGG GCT GAT GAG
Dynamin-like protein 1A (ADL1A)	327	Forward: GTG AAC AAA ATC CAA AGA GCT TG	64
		Reverse: GCC AGT TTC TCG ATC TGT CTC
ROC7 cyclophilin	249	Forward: CCA GGC AAA GAA GTC AAA GGA G	66
		Reverse: TCA CCT CCC TGA ATC ATG AAA CT
Rab GTP-binding protein (Rab5)	225	Forward: CCA TAG GTG CTG CCT TCT TCT C	66
		Reverse: CCA TGT TTG GAT TGC CTT GTG ATT
Vesicle-associated membrane protein 722 (Vamp 722)	356	Forward: GCA AAG CAG GTG GTC TCA GG	64
		Reverse: TTA AGG CTA TTG GCA GGG GCT
Clathrin-binding protein β-adaptin	269	Forward: CTT GGT GAT CTG ATT GGC ATG G	66
		Reverse: ACT TGT GGA ACC TGA AGG GGT C
Rab GTP-binding protein (Rab11)	316	Forward: ATG TTT GTA GGT TAT TAG TCG CTT A	66
		Reverse: CGC TCT TGA CCA GTT GTA TCC CA