Table 2.
Clone IDa | Homologue definition | Organism | GenBank | COBb | E value | IDENTc | SIMd | CAZye | Experimental conditionf | |
J | W | |||||||||
Genes with some function related to cell wall metabolism | ||||||||||
PP_LEa0003N12f | Cobra (Cob) | A. thaliana | NP_568930 | 96 | 0.0 | 78 | 88 | – | 1.80 | 0.56 |
PP_LEa0004K19f | Glucan synthase | O. sativa | NP_916159 | 100 | 7e-75 | 68 | 85 | GT48 | 1.25 | 0.80 |
PP_LEa0005N23f | Galactosyltransferase family protein | A. thaliana | NP_193838 | 99 | 2e-80 | 72 | 86 | GT31 | 1.84 | 0.54 |
PP_LEa0006P14f | Endopolygalacturonase (EndoPG) | P. persica | AAC64184 | 100 | 0.0 | 99 | 100 | GH28 | 3.23 | 0.31 |
PP_LEa0008K15f | Cinnamoyl-CoA reductase (CCR) | A. thaliana | NP_200657 | 89 | 1e-81 | 75 | 88 | – | 1.99 | 0.50 |
PP_LEa0015N02f | Pectate lyase (PL) | A. thaliana | NP_191074 | 100 | 8e-90 | 80 | 92 | PL1 | 1.93 | 0.52 |
PP_LEa0033B07f | Cotton fibre expressed protein 2 | G. hirsutum | AAC33277 | 95 | 2e-18 | 40 | 55 | – | 2.17 | 0.46 |
Genes related to endomembrane traffic | ||||||||||
PP_LEa0001H21f | ER lumen protein retaining receptor 2 (ERD2) | A. thaliana | NP_564326 | 99 | 2e-72 | 75 | 90 | n/a | 1.85 | 0.54 |
PP_LEa0004B09f | Coatomer protein gamma 2-subunit (gamma 2-COP) | O. sativa | BAC84213 | 90 | 9e-160 | 89 | 95 | n/a | 0.85 | 1.17 |
PP_LEa0008F22f | Golgi transport protein SFT2-like | A. thaliana | NP_567749 | 100 | 5e-96 | 78 | 87 | n/a | 1.45 | 0.68 |
PP_LEa0009A08f | SNARE-like protein Vap 27–2 | A. thaliana | NP_172359 | 93 | 7e-46 | 41 | 53 | n/a | 1.24 | 0.81 |
PP_LEa0009I13f | Dynamin-like protein 1A (ADL1A) | G. max | AAB05992 | 99 | 4e-94 | 94 | 97 | n/a | 1.34 | 0.74 |
PP_LEa0009K22f | ROC7 cyclophilin | A. thaliana | NP_200679 | 100 | 7e-89 | 82 | 92 | n/a | 1.74 | 0.57 |
PP_LEa0013L18f | Rab GTP-binding protein (Rab5) | N. tabacum | CAA50609 | 96 | 5e-79 | 89 | 96 | n/a | 1.89 | 0.53 |
PP_LEa0014G24f | Vesicle-associated membrane protein 722 (Vamp 722) | O. sativa | CAD70274 | 99 | 5e-99 | 86 | 94 | n/a | 2.10 | 0.48 |
PP_LEa0017D15f | Clathrin-binding protein β-adaptin | A. thaliana | NP_192877 | 89 | 2e-35 | 75 | 88 | n/a | 0.75 | 1.33 |
PP_LEa0020B02f | Rab GTP-binding protein (Rab11) | G. hirsutum | AAD48018 | 80 | 1e-38 | 85 | 92 | n/a | 1.56 | 0.64 |
PP_LEa0024O23f | GTPase-activating protein Sec23 | A. thaliana | NP_563741 | 86 | 1e-91 | 89 | 94 | n/a | 2.77 | 0.36 |
PP_LEa0030L15f | Secretory carrier membrane protein like (SCAMP-like) | P. sativum | AAC82326 | 96 | 9e-125 | 75 | 88 | n/a | 1.52 | 0.66 |
Nineteen differentially expressed genes were grouped into two physiological processes: cell wall metabolism and endomembrane traffic.
Clone identification according to Clemson University Genomics Institute database (http://www.genome.clemson.edu/projects/peach/est/).
COB, percentage of P. persica sequence length that aligned with the corresponding homologue.
IDENT and
SIM, the identity and similarity percentages, were obtained using the BLOSUM62 matrix (http://www.ncbi.nlm.nih.gov).
CAZy database classification (http://afmb.cnrs-mrs.fr/∼pedro/CAZY).
Expression ratios of juicy/woolly (J) and woolly/juicy (W).