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. 2008 May 3;59(8):1973–1986. doi: 10.1093/jxb/ern069

Table 2.

Selected genes differentially expressed between juicy and woolly fruit

Clone IDa Homologue definition Organism GenBank COBb E value IDENTc SIMd CAZye Experimental conditionf
J W
Genes with some function related to cell wall metabolism
PP_LEa0003N12f Cobra (Cob) A. thaliana NP_568930 96 0.0 78 88 1.80 0.56
PP_LEa0004K19f Glucan synthase O. sativa NP_916159 100 7e-75 68 85 GT48 1.25 0.80
PP_LEa0005N23f Galactosyltransferase family protein A. thaliana NP_193838 99 2e-80 72 86 GT31 1.84 0.54
PP_LEa0006P14f Endopolygalacturonase (EndoPG) P. persica AAC64184 100 0.0 99 100 GH28 3.23 0.31
PP_LEa0008K15f Cinnamoyl-CoA reductase (CCR) A. thaliana NP_200657 89 1e-81 75 88 1.99 0.50
PP_LEa0015N02f Pectate lyase (PL) A. thaliana NP_191074 100 8e-90 80 92 PL1 1.93 0.52
PP_LEa0033B07f Cotton fibre expressed protein 2 G. hirsutum AAC33277 95 2e-18 40 55 2.17 0.46
Genes related to endomembrane traffic
PP_LEa0001H21f ER lumen protein retaining receptor 2 (ERD2) A. thaliana NP_564326 99 2e-72 75 90 n/a 1.85 0.54
PP_LEa0004B09f Coatomer protein gamma 2-subunit (gamma 2-COP) O. sativa BAC84213 90 9e-160 89 95 n/a 0.85 1.17
PP_LEa0008F22f Golgi transport protein SFT2-like A. thaliana NP_567749 100 5e-96 78 87 n/a 1.45 0.68
PP_LEa0009A08f SNARE-like protein Vap 27–2 A. thaliana NP_172359 93 7e-46 41 53 n/a 1.24 0.81
PP_LEa0009I13f Dynamin-like protein 1A (ADL1A) G. max AAB05992 99 4e-94 94 97 n/a 1.34 0.74
PP_LEa0009K22f ROC7 cyclophilin A. thaliana NP_200679 100 7e-89 82 92 n/a 1.74 0.57
PP_LEa0013L18f Rab GTP-binding protein (Rab5) N. tabacum CAA50609 96 5e-79 89 96 n/a 1.89 0.53
PP_LEa0014G24f Vesicle-associated membrane protein 722 (Vamp 722) O. sativa CAD70274 99 5e-99 86 94 n/a 2.10 0.48
PP_LEa0017D15f Clathrin-binding protein β-adaptin A. thaliana NP_192877 89 2e-35 75 88 n/a 0.75 1.33
PP_LEa0020B02f Rab GTP-binding protein (Rab11) G. hirsutum AAD48018 80 1e-38 85 92 n/a 1.56 0.64
PP_LEa0024O23f GTPase-activating protein Sec23 A. thaliana NP_563741 86 1e-91 89 94 n/a 2.77 0.36
PP_LEa0030L15f Secretory carrier membrane protein like (SCAMP-like) P. sativum AAC82326 96 9e-125 75 88 n/a 1.52 0.66

Nineteen differentially expressed genes were grouped into two physiological processes: cell wall metabolism and endomembrane traffic.

a

Clone identification according to Clemson University Genomics Institute database (http://www.genome.clemson.edu/projects/peach/est/).

b

COB, percentage of P. persica sequence length that aligned with the corresponding homologue.

c

IDENT and

d

SIM, the identity and similarity percentages, were obtained using the BLOSUM62 matrix (http://www.ncbi.nlm.nih.gov).

e

CAZy database classification (http://afmb.cnrs-mrs.fr/∼pedro/CAZY).

f

Expression ratios of juicy/woolly (J) and woolly/juicy (W).