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Inhibition of MDH disaggregation by sulfate and centrifugation. (ClpB+KJE)-mediated MDH reactivation as in Fig. 1 was measured after 270 min. Addition of 100 mM (NH4)2SO4 or centrifugation (3 min, 16,000 × g) was performed at indicated time points. Inhibition of reactivation was expressed as percent loss of recovered MDH activity as compared with the activity without inhibition at 270 min.
