Figure 3.

Modulation of interaction and ClpB-induced conformational changes in heat-aggregated MDH. Time-dependent change in bis-ANS binding (A) and Congo red binding (B) to heat-aggregated MDH after incubation with ATP, ClpB and/or KJE as in Fig. 1B. bis-ANS and Congo red binding to aggregated MDH before chaperone addition is set as 100%; baseline binding levels in native MDH is set as 0%. (C) Interaction of ClpB₆ with MDH aggregates and nucleotides by Trp fluorescence. Effects of increasing concentrations of aggregated MDH on the intrinsic fluorescence intensity of ClpB₆ (83 nM) without or with 3 mM ATP or ADP.