Figure 2.

Steady-state levels of wild-type and mutant Src proteins. (A) (Upper) Src−/− MEFs were transfected with pLNCX vector expressing c-Src, v-Src, Y527F, E378G, Y416F, or K295R forms of Src, or pLNCX alone. Cells were harvested after 48 hr, and 50 μg of whole-cell lysate from each transfection was separated by SDS/PAGE, transferred to PVDF membrane, and probed with mAb 327. (Lower) The same membrane reprobed with anti-actin antibody C11. (B) Quantitation of the results shown in A by using densitometry.