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. 2008 Jun 18;3(6):e2430. doi: 10.1371/journal.pone.0002430

Figure 6. Math6 can function as a transcriptional repressor in vitro.

Figure 6

A high background reporter plasmid consisting of five tandem copies of the Gal4 UAS upstream of the prolactin minimal promoter driving luciferase was cotransfected with increasing amounts of the plasmid expressing a fusion protein comprised of the GAL4 DNA binding domain (GAL4DBD) and full length Math6. Additionally, a GAL4DBD-Retinoblastoma Large Pocket (RbLP) and a GAL4-p300 (fragment 1737–2414 nt) fusion proteins were included as controls for repression and activation, respectively. Relative luciferase activities were calculated with the activity of cells transfected with the lowest amount of the GAL4DBD alone set at 1. All data are mean±SEM from transfections performed in duplicates on at least 4 occasions. * P<0.05. **P<0.01 as compared to equivalent amount of GAL4DBD vector (Student's t-test).