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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Neurobiol Dis. 2008 Feb 9;30(2):201–211. doi: 10.1016/j.nbd.2008.01.006

Figure 4.

Figure 4

PP2 applied just prior to thrombin decreases thrombin induced injury to cultured striatal neurons. Panels A–D show striatal neurons exposed to PP2 (0.3 µM) alone for 24h and labeled for MAP-2 (A), TUNEL (B), DAPI (C), and the overlay (D). Application of thrombin (30U/ml) produced injury to the neurons labeled for MAP-2 (E), TUNEL (F), DAPI (G) and the overlay (H). Application of 0.1 µM PP2 ten minutes prior to thrombin (30 U/ml) has a marginal effect 24h later on TUNEL stained (J) MAP-2 (I) and DAPI (K) positive striatal neurons (L, overlay). Application of 0.3 µM PP2 ten minutes prior to thrombin (30 U/ml) markedly decreases the numbers of TUNEL stained cells (N) that were MAP-2 (M) and DAPI (O) positive striatal neurons (P, overlay). Scale bar: A–P, 100 µm.