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Hybridization and restriction enzyme cleavage of different combinations of labeled and unlabeled oligonucleotides. Restriction digest analyses of the hybrid DNA were performed with the restriction enzymes HindIII and KpnI either alone or in combination. The restriction sites lead to symmetrically cleaved fragments (2x12 mers, 1x16 mer for double digest). The filled circles correspond to labeled DNA. Q01–03, intensities of DNA hybrids; Qi, intensities of cleavage products.
