Figure 4.

Difference Δp in the probability per gate that 15-kbp and 33.5-kbp molecules skip to the right (blue). Extrapolated number of bands n_C that could be detected between 15 kbp and 33.5 kbp in a 10-cm sieve (red). Lines are theoretical predictions for Δp (blue) and n_C (red). The theoretical curve of Δp is taken from Eq. 1 of ref. 6, substituting in the two values of D/va, and calculating p_R and p_L for both fragments: Δp = [p_R(15 kbp) − p_L(15 kbp)] − [p_R(33.5 kbp) − p_L(33.5 kbp)]. Theoretical curve for band capacity n_C uses variance σ² = N [p_R(1 − p_R) + p_L(1 − p_L) + p_Rp_L)], given in ref. 6, where N = 12,500 gates.